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Intramuscular (IM) injection delivers the peptide directly into muscle tissue using a longer needle at a 90-degree angle without pinching

Briefly, cells plated at a density of 2 10 5 cells/cm 2 in 12-well tissue culture-treated polystyrene plates, were first grown for 2 weeks in Williams E medium (Invitrogen, Cergy-Pontoise, France) supplemented with 10% (vol/vol) fetal calf serum (FCS) (Cytiva, Velizy-Villacoublay, France), 100 IU/ml penicillin, 100 g/ml streptomycin, 5 g/ml bovine insulin (SigmaAldrich, Saint-Quentin-Fallavier, France), 2 mM glutamine (Invitrogen) and 50 M hydrocortisone hemisuccinate (Upjohn, Paris La Dfense, France)

Eligibility typically depends on BMI guidelines and medical history, which are evaluated during an online consultation

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